MYL6 (phospho S19/20) polyclonal antibody
产品名称： MYL6 (phospho S19/20) polyclonal antibody
英文名称： MYL6 (phospho S19/20) polyclonal antibody
产品编号： PAB9961
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic phosphopeptide of MYL6.
- Immunogen:
- Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S19/S20 of human MYL6.
- Host:
- Rabbit
- Reactivity:
- Human
- Specificity:
- This antibody is phosphospecific and detect monophosphorylated and diphosphorylated forms of the protein. This phosphospecific polyclonal antibody is specific to the phosphorylated pS19/pS20 form of the protein, depending on the source origin of the protein.
- Form:
- Liquid
- Storage Buffer:
- In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Antibody Reactive Against Synthetic Peptide.
- Recommend Usage:
- ELISA (1:120000-1:135000)
Western Blot (1:1000-1:5000)
Immunohistochemistry (2.5 ug/mL)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Applications
- Western Blot (Cell lysate)
- MYL6 (phospho S19/20) polyclonal antibody (Cat # PAB9961) was used at a 1 : 5000 dilution to detect myosin light chain by Western blot.
Either 13 or 20 uL of a mouse cardiac myocyte lysate was loaded on a 4-20% Criterion gel for SDS-PAGE. Samples were either mock-treated or CLA-treated, as indicated. After washing, a 1 : 5,000 dilution of HRP conjugated Gt-a-Rabbit IgG preceded color development using Amersham's substrate system.
Other detection methods will yield similar results.
Data courtesy of the Alliance for Cellular Signaling (http://www.signaling-gateway.org).
- Western Blot (Cell lysate)
- MYL6 (phospho S19/20) polyclonal antibody (Cat # PAB9961) was used at a 1 : 1000 dilution to detect myosin light chain by Western blot on NIH/3T3 cell lysates.
A standard urea/glycerol gel without SDS was used to separate phospho forms of regulatory light chain according to mass to charge ratios.
In Panel A, reactivity of MYL6 (phospho S19/20) polyclonal antibody (Cat # PAB9961) is shown.
In Panel B, reactivity of commercially available pan reactive antibody that detects both unphosphorylated and phosphorylated forms of regulatory light chain is shown.The phosphospecific antibody detects both monophosphorylated (pSer20 Mono-P-RLC) and diphosphorylated (pThr19-pSer20 Di-P-RLC) regulatory light chain.
Personal communication. J. Stull. UT Southwestern Medical Center.
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Immunohistochemical staining with MYL6 (phospho S19/20) polyclonal antibody (Cat # PAB9961) was used at 2.5 ug/mL to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides.
This image shows strong staining of both vascular and myometrial smooth muscle cells of the uterus.
Tissue was formalin-fixed and paraffin embedded.
The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Personal Communication, Tina Roush, LifeSpanBiosciences, Seattle, WA.
- Entrez GeneID:
- 4637
- Gene Name:
- MYL6
- Gene Alias:
- ESMLC,LC17-GI,LC17-NM,LC17A,LC17B,MLC1SM,MLC3NM,MLC3SM
- Gene Description:
- myosin, light chain 6, alkali, smooth muscle and non-muscle
- Omim ID:
- 609931
- Gene Ontology:
- Hyperlink
- Gene Summary:
- Myosin is a hexameric ATPase cellular motor protein. It is composed of two heavy chains, two nonphosphorylatable alkali light chains, and two phosphorylatable regulatory light chains. This gene encodes a myosin alkali light chain that is expressed in smooth muscle and non-muscle tissues. Genomic sequences representing several pseudogenes have been described and two transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq
- Other Designations:
- myosin, light polypeptide 6, alkali, smooth muscle and non-muscle,smooth muscle and non-muscle myosin alkali light chain
- Gene Pathway