Anti-Phospho-Akt (Thr308) (Clone NL50) Rabbit mAb-其它资料-资讯-生物在线

Anti-Phospho-Akt (Thr308) (Clone NL50) Rabbit mAb

作者:上海康成生物工程有限公司 2008-01-30T00:00 (访问量:2014)

Anti-Phospho-Akt (Thr308) (Clone NL50) Rabbit mAb

 

Catalog No: KC-5A03

Size: 100 µl (10 Western mini-blots)

 


Applications

Species Cross-Reactivity

Molecular Wt.

Source

W, IHC, F

H, M, R

60 kDa

Rabbit

 

Western blot analysis of extracts from NIH/3T3 cells, untreated, PDGF-treated, or PDGF-treated after treatment with wortmannin and/or rapamycin as indicated, using Phospho-Akt (Thr308) (NL50) Rabbit MAb.

Background:

Akt, also referred to as PKB or Rac, plays a critical role in controlling the balance between survival and apoptosis. This protein kinase is activated by insulin and various growth and survival factors, and functions in a wortmannin-sensitive pathway involving PI3 kinase. Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1, and by phosphorylation within the carboxy-terminus at Ser473. Akt promotes cell survival by inhibiting apoptosis through its ability to phosphorylate and inactivate several targets, including Bad, Forkhead transcription factors and caspase-9. PTEN phosphatase is a major negative regulator of the PI3 kinase/Akt signaling pathway. LY294002 is a specific PI3 kinase inhibitor. One of the essential functions of Akt is the regulation of glycogen synthesis through phosphorylation and inactivation of glycogen synthase kinase-3 alpha and beta. Akt may also play a role in insulin stimulation of glucose transport.

 

In addition to its role in survival and glycogen synthesis, Akt is involved in cell cycle regulation by preventing GSK3 beta-mediated phosphorylation and degradation of cyclin D1, and by negatively regulating the cyclin-dependent kinase inhibitors p27 KIP and p21 WAF1. Akt also plays a critical role in cell growth by directly phosphorylating the mammalian target of rapamycin, mTOR, but more importantly through phosphorylation and in activation of tuberin (TSC2), an mTOR inhibitor. Inhibition of mTOR stops the protein synthesis machinery due to inactivation of its effec-tor, p70 S6 kinase, and activation of the eukaryotic initiation factor, 4E binding protein 1 (4E-EP1), an inhibitor of translation.

 

Akt1 shares 81% and 83% amino acid identity with Akt2 andAkt3, respectively.Although the three isoforms show broad tissue distribution, Akt1 is the most ubiquitously expressed. Akt2 is expressed at a lower level than Akt1 except in insulin-responsive tissues where it predominates. In particular, Akt2 is more abundant and more highly activated than Akt1 in adipocytes. Akt3 is expressed at the lowest level except in testes and brain. The C-terminal sequence of the three isoforms are shown below. Note that the phosphorylation site slightly differs between the three isoforms.Thus the phosphorylation site Ser473 of Akt1 corresponds to Ser474 in Akt2 and Ser472 in Akt3 respectively.

 

Specificity/Sensitivity:

Phospho-Akt (Thr308) (NL50) Rabbit Monoclonal Antibody detects endoge-nous levels of Akt only when phosphorylated at threo-nine 308.

 

Source/Purification:

Monoclonal antibodies are produced by immunizing rabbits with a synthetic phosphopeptide (KLH coupled) corresponding to residues around Thr308 of mouse Akt.

 

Storage:

Each vial contains 100ul affinity purified rabbit anti-phospho-Akt antibody in a special stabilizer. Real time studies that this special stabilizer helped retain most of antibody activity for 24 months while store at 4℃.

 

Recommended Antibody Dilutions:

Western blotting

1:1000

Immunohistochemistry (Paraffin)

1:80

Flow cytometry

1:100

 

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